ca. 1610

[Possible/Probable] “Confinement of Jews into a Ghetto” issued by Duke Vincenzo Gonzaga [Duchy of Mantua; Present-day Italy] 

Commentary from other sources:
1) In Mantua, Jews were required to live in a walled ghetto. The gates of the ghetto were locked at sunset and unlocked at dawn.

Morgan, Howard: So Deeply Scarred: A History of “Christian” Anti-Semitism (2016)