ca. 1610

“Confinement of Jews into a Ghetto” issued by Duke Vincenzo Gonzaga [Duchy of Mantua; Present-day Italy] [Unconfirmed]

Commentary from other sources:
1) In Mantua, Jews were required to live in a walled ghetto. The gates of the ghetto were locked at sunset and unlocked at dawn.
Morgan, Howard: So Deeply Scarred: A History of “Christian” Anti-Semitism (2016)